The interview will be broadcast on December 27.
The pair's longtime bromance was on full display as Harry interviewed the former USA president, trading quips in a teaser released on Monday morning (AEST).
"Do I need to speak faster?" Obama interrupts, shooting a smirk at the prince.
"If you start using long pauses between the answers, you're probably gonna get 'the face, '" Prince Harry responded jokingly.
A weekly overview of the chatter from Kensington Palace and beyond.
The bromance between Britain's Prince Harry and former United States president Barack Obama has continued during an interview for British radio.
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When Harry showed him what he meant, Obama said: "I don't want to see that face".
Kensington Palace said the conversation features Obama talking about his plans to focus on cultivating the next generation of leadership through the Obama Foundation. They were pictured laughing together at this year's Invictus Games in Canada.
Last year, Obama and his wife, Michelle, challenged Harry and his British competitors "to bring it" ahead of the event in Orlando.
Response to the royal and presidential interview has been great and some fans pointed out that "Harry and Barry" made quite the team. During that meeting, they discussed shared interests around veterans and empowering young people.
Since Obama left office, he has met Harry on several occasions, including a stop-over to Kensington Palace in May during his tour of Europe, and during Obama's Foundation Summit in October. Some have even stated they can't wait until December 27, 2017, to see it.